Effects of a Low Dose X-Ray Irradiation and Simulated Microgravity on Physiological, Oxidative Stress Biomarker and Metabolomics Adaptations: OAJBS Publishers

Effects of a Low Dose X-Ray Irradiation and Simulated Microgravity on Physiological, Oxidative Stress Biomarker and Metabolomics Adaptations by P Chowdhury* in Open Access Journal of Biomedical Science (OAJBS)

This aim of this study is to utilize a ground-based rat model of hindlimb unloading and examine the effect of 0.3 GY radiation on oxidative stress biomarker and metabolomics adaptations.

Methods: For this study, we have used four groups (four rats per group) for three weeks in the following order: 1) non-suspended control (CON), 2) irradiated non-suspended (IR), 3) irradiated plus hindlimb suspended (IR+HLS) and 4) hindlimb suspended (HLS). IR and IR+HLS groups were exposed to five sessions of X-ray irradiation (0.3 Gy each daily). Body weight, food and water intakes measured daily. At term, the animals were anesthetized with isoflurane, blood was drawn in a syringe to collect serum for protein and metabolomics assays. Lipid peroxidation was measured on collected tissue samples. Data were pooled, analysed and tested statistically using one way ANOVA.

Results: CON animals gained weight faster than IR + HLS or HLS group, whereas weight gain in IR group was closely similar to control group. Food consumption and water intakes were not significantly different between the groups. Serum insulin levels and tissue MDA levels were variable among the groups with no significant differences. Serum metabolites showed significant differences in aspartate and gammaglutamyl-glutamine concentrations between HLS and IR + HLS groups as compared to control groups although many of the other metabolomes measured were not significantly changed. Summary: HLS and HLS +IR animals showed reduced growth as compared to control and IR groups. There are insignificant but variable changes in tissue oxidative parameters and insulin levels. Significant changes in some specific serum metabolites were observed.

Conclusion: Decreased body weight gain in HLS and HLS + IR groups do not appear to be due to decrease in food intake. Changes in specific metabolites in HLS and HLS + IR group appear to be a stress related response. Overall, the data reflects variability due to altered experimental design and low radiation effect (Supported by a grant from Arkansas Space Grant Consortium).
https://biomedscis.com/fulltext/effects-of-a-low-dose-x-ray-irradiation-and-simulated-microgravity-on-physiological.ID.000330.php

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