Open Access Journal of Biomedical Science | Biomedscis

Comparison of 70% Alcohol Disinfection and Sodium Hypochlorite Acid Disinfection on Kitchen Knife Blades, Handles and Cutting Boards by Naomi Katayama* in Open Access Journal of Biomedical Science (OAJBS) Purpose: In hygiene management in the kitchen, it is important to disinfect kitchen knives and cutting boards, which are often touched by human hands and food. It has become with COVID-19, and there is a demand for even more hygienic kitchen utensils. However, had to lack of supplies, there were times when it was difficult of obtain 70% alcohol, so the kitchen was temporarily disinfected with sodium hypochlorite. This time, we compared the results of ATP wiping inspections on the blade and handle (stainless steel) of kitchen knives and cutting board (plastic) that were disinfected with sodium hypochlorite obtained by electrolysis of salt water or 70% alcohol disinfection. We will report that obtained the target basics-hygiene data. Materials and Methods: Using 6 kitchen knives, ...

Improving of Cryopreservation Quality of Spermatogonial Stem Cells after Freezing-Thawing by Melatonin by Farzane Farokhi* in Open Access Journal of Biomedical Science (OAJBS) Cryopreservation of spermatogonial stem cells (SSCs) is an advantageous method to restore fertility in young boys’ cancer survivors. Recently, for reduction of SSCs cryoinjering various antioxidants have been used. The purpose of this study was to evaluate antioxidant effects of melatonin in frozen-thawed SSCs. Spermatogonial stem cells were isolated from testes of neonate mice (3-6 days old) and their purities were measured by flow cytometry with GFRα1. After culturing, the cells were frozen in two groups 1- control and 2- melatonin (100μM) for one month. Finally, the cell viability, colonization rate, intracellular ROS were evaluated after freezing thawing. Melatonin increased the viability, colonization of SSCs, and decreased intracellular ROS. The results of this study show that melatonin with antioxidan...

Practical Recommendations on the Management of Issues Related to the Levonorgestrel 52mg Intrauterine System: A Delphi Panel by Thais Emy Ushikusa* in Open Access Journal of Biomedical Science (OAJBS)   Objective: To gather the routine practice of Brazilian physicians with large experience on the Levonorgestrel 52mg intrauterine system (LNG IUS) use in order to generate practical recommendations on how to manage common aspects related to it. Material and Methods: Twenty gynecologists with large experience with LNG IUS answered 39 questions grouped into five main topics: exams before the procedure, technical aspects of the device placement, practical recommendations, irregular bleeding, and pregnancy. A second round of questions was carried when responses were very divergent. Results: Eighteen panelists responded to the questions. Recommendations that had more than 90% of agreement were i) the gynecological exam is the most important assessment before the insertion of LNG I...